WhiteRotV1, Main, Exploration, bibRecord, 000296

Down-regulation of pyruvate decarboxylase gene of white-rot fungus Phlebia sp. MG-60 modify the metabolism of sugars and productivity of extracellular peroxidase activity.

Identifieur interne : 000296 ( Main/Exploration ); précédent : 000295; suivant : 000297

Down-regulation of pyruvate decarboxylase gene of white-rot fungus Phlebia sp. MG-60 modify the metabolism of sugars and productivity of extracellular peroxidase activity.

Auteurs : Taichi Motoda [Japon] ; Megumi Yamaguchi [Japon] ; Taku Tsuyama [Japon] ; Ichiro Kamei [Japon]

Source :

Journal of bioscience and bioengineering [ 1347-4421 ] ; 2019.

RBID : pubmed:30007481

Descripteurs français

English descriptors

KwdEn :
- Basidiomycota (genetics), Basidiomycota (metabolism), Carbohydrate Metabolism (genetics), Down-Regulation (genetics), Ethanol (metabolism), Fermentation (MeSH), Gene Expression Regulation, Enzymologic (MeSH), Gene Expression Regulation, Fungal (MeSH), Gene Silencing (MeSH), Lignin (metabolism), Metabolic Engineering (methods), Organisms, Genetically Modified (MeSH), Peroxidases (genetics), Peroxidases (metabolism), Pyruvate Decarboxylase (genetics), Pyruvate Decarboxylase (metabolism), Sugars (metabolism), Xylitol (metabolism), Xylose (metabolism).
MESH :
- chemical , genetics : Peroxidases, Pyruvate Decarboxylase.
- chemical , metabolism : Ethanol, Lignin, Peroxidases, Pyruvate Decarboxylase, Sugars, Xylitol, Xylose.
- genetics : Basidiomycota, Carbohydrate Metabolism, Down-Regulation.
- metabolism : Basidiomycota.
- methods : Metabolic Engineering.
- Fermentation, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Fungal, Gene Silencing, Organisms, Genetically Modified.

Abstract

Ethanologenic white-rot fungus Phlebia sp. MG-60-P2 produces ethanol directly from several lignocelluloses. Efficient gene silencing methods are needed for metabolic engineering of this fungus for biorefinery use. In this study, we evaluated the effectiveness of RNAi-mediated silencing of the pyruvate decarboxylase gene of Phlebia sp. MG-60-P2 (MGpdc1). The RNAi lines generated showed a variety of suppression levels of ethanol production and MGpdc1 expression, and two selected strains led to different metabolic fluxes, resulting in rapid accumulation of xylitol from xylose. Knockdown lines KD2 and KD10 showed different strength of silencing. The moderate-inhibition line (KD10) showed faster xylitol accumulation from xylose than the severe-inhibition line (KD2). KD2, KD10 and knockout line KO77 showed higher extracellular peroxidase activity than the wild-type. Gene silencing using RNAi for MGpdc1 in the ethanologenic white-rot fungus Phlebia sp. MG-60-P2 is an effective first step in metabolic engineering to produce other chemicals besides ethanol. This high efficiency of transformation and silencing effect will make it possible to cotransform with multiple expression vectors which enhance the minor metabolic pathway or introduce exogenous metabolic reaction in Phlebia sp. MG-60-P2.

DOI: 10.1016/j.jbiosc.2018.06.017
PubMed: 30007481

Affiliations:

Japon

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000384
to stream Main, to step Curation: 000384

Le document en format XML

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<term>Down-Regulation (genetics)</term>
<term>Ethanol (metabolism)</term>
<term>Fermentation (MeSH)</term>
<term>Gene Expression Regulation, Enzymologic (MeSH)</term>
<term>Gene Expression Regulation, Fungal (MeSH)</term>
<term>Gene Silencing (MeSH)</term>
<term>Lignin (metabolism)</term>
<term>Metabolic Engineering (methods)</term>
<term>Organisms, Genetically Modified (MeSH)</term>
<term>Peroxidases (genetics)</term>
<term>Peroxidases (metabolism)</term>
<term>Pyruvate Decarboxylase (genetics)</term>
<term>Pyruvate Decarboxylase (metabolism)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (génétique)</term>
<term>Basidiomycota (métabolisme)</term>
<term>Extinction de l'expression des gènes (MeSH)</term>
<term>Fermentation (MeSH)</term>
<term>Génie métabolique (méthodes)</term>
<term>Lignine (métabolisme)</term>
<term>Métabolisme glucidique (génétique)</term>
<term>Organismes génétiquement modifiés (MeSH)</term>
<term>Peroxidases (génétique)</term>
<term>Peroxidases (métabolisme)</term>
<term>Pyruvate decarboxylase (génétique)</term>
<term>Pyruvate decarboxylase (métabolisme)</term>
<term>Régulation de l'expression des gènes codant pour des enzymes (MeSH)</term>
<term>Régulation de l'expression des gènes fongiques (MeSH)</term>
<term>Régulation négative (génétique)</term>
<term>Sucres (métabolisme)</term>
<term>Xylitol (métabolisme)</term>
<term>Xylose (métabolisme)</term>
<term>Éthanol (métabolisme)</term>
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<term>Pyruvate Decarboxylase</term>
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<term>Lignin</term>
<term>Peroxidases</term>
<term>Pyruvate Decarboxylase</term>
<term>Sugars</term>
<term>Xylitol</term>
<term>Xylose</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term>
<term>Carbohydrate Metabolism</term>
<term>Down-Regulation</term>
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<term>Métabolisme glucidique</term>
<term>Peroxidases</term>
<term>Pyruvate decarboxylase</term>
<term>Régulation négative</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Metabolic Engineering</term>
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<term>Lignine</term>
<term>Peroxidases</term>
<term>Pyruvate decarboxylase</term>
<term>Sucres</term>
<term>Xylitol</term>
<term>Xylose</term>
<term>Éthanol</term>
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<term>Gene Expression Regulation, Enzymologic</term>
<term>Gene Expression Regulation, Fungal</term>
<term>Gene Silencing</term>
<term>Organisms, Genetically Modified</term>
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<term>Fermentation</term>
<term>Organismes génétiquement modifiés</term>
<term>Régulation de l'expression des gènes codant pour des enzymes</term>
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<front><div type="abstract" xml:lang="en">Ethanologenic white-rot fungus Phlebia sp. MG-60-P2 produces ethanol directly from several lignocelluloses. Efficient gene silencing methods are needed for metabolic engineering of this fungus for biorefinery use. In this study, we evaluated the effectiveness of RNAi-mediated silencing of the pyruvate decarboxylase gene of Phlebia sp. MG-60-P2 (MGpdc1). The RNAi lines generated showed a variety of suppression levels of ethanol production and MGpdc1 expression, and two selected strains led to different metabolic fluxes, resulting in rapid accumulation of xylitol from xylose. Knockdown lines KD2 and KD10 showed different strength of silencing. The moderate-inhibition line (KD10) showed faster xylitol accumulation from xylose than the severe-inhibition line (KD2). KD2, KD10 and knockout line KO77 showed higher extracellular peroxidase activity than the wild-type. Gene silencing using RNAi for MGpdc1 in the ethanologenic white-rot fungus Phlebia sp. MG-60-P2 is an effective first step in metabolic engineering to produce other chemicals besides ethanol. This high efficiency of transformation and silencing effect will make it possible to cotransform with multiple expression vectors which enhance the minor metabolic pathway or introduce exogenous metabolic reaction in Phlebia sp. MG-60-P2.</div>
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<ArticleTitle>Down-regulation of pyruvate decarboxylase gene of white-rot fungus Phlebia sp. MG-60 modify the metabolism of sugars and productivity of extracellular peroxidase activity.</ArticleTitle>
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<Abstract><AbstractText>Ethanologenic white-rot fungus Phlebia sp. MG-60-P2 produces ethanol directly from several lignocelluloses. Efficient gene silencing methods are needed for metabolic engineering of this fungus for biorefinery use. In this study, we evaluated the effectiveness of RNAi-mediated silencing of the pyruvate decarboxylase gene of Phlebia sp. MG-60-P2 (MGpdc1). The RNAi lines generated showed a variety of suppression levels of ethanol production and MGpdc1 expression, and two selected strains led to different metabolic fluxes, resulting in rapid accumulation of xylitol from xylose. Knockdown lines KD2 and KD10 showed different strength of silencing. The moderate-inhibition line (KD10) showed faster xylitol accumulation from xylose than the severe-inhibition line (KD2). KD2, KD10 and knockout line KO77 showed higher extracellular peroxidase activity than the wild-type. Gene silencing using RNAi for MGpdc1 in the ethanologenic white-rot fungus Phlebia sp. MG-60-P2 is an effective first step in metabolic engineering to produce other chemicals besides ethanol. This high efficiency of transformation and silencing effect will make it possible to cotransform with multiple expression vectors which enhance the minor metabolic pathway or introduce exogenous metabolic reaction in Phlebia sp. MG-60-P2.</AbstractText>
<CopyrightInformation>Copyright © 2018 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</CopyrightInformation>
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<ForeName>Taichi</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-kibanadai-nishi, Miyazaki 889-2192, Japan. Electronic address: gb13048@student.miyazaki-u.ac.jp.</Affiliation>
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<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
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<MeshHeading><DescriptorName UI="D011767" MajorTopicYN="N">Pyruvate Decarboxylase</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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<MeshHeading><DescriptorName UI="D000073893" MajorTopicYN="N">Sugars</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
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<MeshHeading><DescriptorName UI="D014993" MajorTopicYN="N">Xylitol</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014994" MajorTopicYN="N">Xylose</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
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<Keyword MajorTopicYN="N">Extracellular peroxidase</Keyword>
<Keyword MajorTopicYN="N">Fermentation</Keyword>
<Keyword MajorTopicYN="N">Pyruvate decarboxylase</Keyword>
<Keyword MajorTopicYN="N">RNAi</Keyword>
<Keyword MajorTopicYN="N">White-rot fungi</Keyword>
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<ArticleId IdType="doi">10.1016/j.jbiosc.2018.06.017</ArticleId>
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<affiliations><list><country><li>Japon</li>
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<name sortKey="Kamei, Ichiro" sort="Kamei, Ichiro" uniqKey="Kamei I" first="Ichiro" last="Kamei">Ichiro Kamei</name>
<name sortKey="Tsuyama, Taku" sort="Tsuyama, Taku" uniqKey="Tsuyama T" first="Taku" last="Tsuyama">Taku Tsuyama</name>
<name sortKey="Yamaguchi, Megumi" sort="Yamaguchi, Megumi" uniqKey="Yamaguchi M" first="Megumi" last="Yamaguchi">Megumi Yamaguchi</name>
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Down-regulation of pyruvate decarboxylase gene of white-rot fungus Phlebia sp. MG-60 modify the metabolism of sugars and productivity of extracellular peroxidase activity.

Down-regulation of pyruvate decarboxylase gene of white-rot fungus Phlebia sp. MG-60 modify the metabolism of sugars and productivity of extracellular peroxidase activity.

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